منابع مشابه
Common location of determinants in initiator transfer RNAs for initiator-elongator discrimination in bacteria and in eukaryotes.
Initiator tRNAs are used exclusively for initiation of protein synthesis and not for elongation. We show that both Escherichia coli and eukaryotic initiator tRNAs have negative determinants, at the same positions, that block their activity in elongation. The primary negative determinant in E. coli initiator tRNA is the C1xA72 mismatch at the end of the acceptor stem. The primary negative determ...
متن کاملThe nucleotide sequences of the initiator transfer RNAs from bean cytoplasm and chloroplasts.
The initiator tRNAsMet from the cytoplasm and chloroplasts of Phaseolus vulgaris have been purified and sequenced. The sequence of bean cytoplasmic initiator tRNAiMet is : pA-U-C-A-G-A-G-U-m1G-m2G-C-G-C-A-G-C-G-G-A-A-G-C-G-U-m2G-G-U-G-G-G2-C-C-C-A-U-t6A-A-C-C-C-A-C-A-G-m7G-D-m5C-C-C-A-G-G-A-psi-C-G-m1A-A-A-C-C-U-Gm-G-C-U-C-U-G-A-U-A-C-C-AOH. The sequence of bean cytoplasmic tRNAiMet is almost i...
متن کاملMethionine transfer RNAF: the initiator transfer RNA for hemoglobin biosynthesis.
This paper presents evidence suggesting that methionyltRNAp is the initiator tRNA for hemoglobin biosynthesis. Our experimental approach was as follows. If the mechanism of mammalian protein synthesis is similar to the mechanism in bacteria, then a tRNA species should be present in the cytoplasm of reticulocytes which has the unique property of being recognized only by its own "initiation" fact...
متن کاملBacterial transfer RNAs.
Transfer RNA is an essential adapter molecule that is found across all three domains of life. The primary role of transfer RNA resides in its critical involvement in the accurate translation of messenger RNA codons during protein synthesis and, therefore, ultimately in the determination of cellular gene expression. This review aims to bring together the results of intensive investigations into ...
متن کاملInitiator Methionine Transfer Ribonucleic Acid from Wheat Embryo
Initiator methionine tRNA (tRNAIMet) species from wheat embryo has been purified by fractionation on a benzoylated DEAE-cellulose column followed by chromatography on a DEAE-Sephadex column. Transfer RNAIMet can be aminoacylated by the homologous enzyme as well as by Escherichia coli aminoacyl-tRNA synthetase. Unlike other eukaryotic cytoplasmic initiator tRNAs, the wheat embryo initiator tRNA ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1994
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.176.3.547-552.1994